| 川芎嗪对人肾癌细胞增殖及凋亡的影响 |
| 投稿时间：2016-12-02 修订日期：2017-01-11 点此下载全文 |
| 引用本文：亓俊华,聂刚,徐秀鹏,周润,姚伟,毛青,罗鹏程.川芎嗪对人肾癌细胞增殖及凋亡的影响[J].医学研究杂志,2017,46(9):96-99 |
| DOI：
10.11969/j.issn.1673-548X.2017.09.026 |
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| 基金项目:黄石市科技计划项目（2015B046-7） |
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| 中文摘要:目的 分析不同浓度川芎嗪（tetramethylpyrazine，TMP）对人肾癌细胞系（786-O）增殖及凋亡的影响，并探讨其分子机制。方法 体外培养786-O细胞，与不同浓度川芎嗪溶液（0.5mmol/L、5mmol/L）及溶剂组（DMSO组）作用24、48和72h后，四甲基偶氮唑盐比色法测定细胞增殖率；细胞计数法检测药物作用48h后的细胞数量变化；DAPI染色观察药物作用48h后细胞核形态；半定量RT-PCR技术和Western blot法检测药物作用48h后凋亡相关基因在转录和翻译水平的改变。结果 随着川芎嗪药物浓度的增加，786-O细胞增殖率显著降低，凋亡率增加，细胞数量也显著减少，且胞核出现皱缩或肿胀，核裂增多；凋亡相关基因AIF、caspase-3在转录及翻译水平上均显著增强，无论与空白对照组还是溶剂对照组比较，差异有统计学意义（P<0.05）。结论 本研究证实TMP可以显著抑制人肾癌细胞系786-O细胞的增殖，促进其凋亡，为中药抗癌方面的研究提供实验室证据。 |
| 中文关键词:川芎嗪 细胞增殖 细胞凋亡 人肾癌细胞系 |
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| Effect of Tetramethylpyrazine on Proliferation and Apoptosis of Human Renal Cell Carcinoma Cells |
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| Abstract:Objective To detect the effects of tetramethylpyrazine (TMP) in different concentrations on proliferation and apoptosis of human renal cell carcinoma cell line (786-O) in vitro and the related molecular mechanism. Methods The 786-O cells were cultured in different concentrations of TMP and DMSO for 24、48、72h. The growth rate of 786-O were detected by MTT assay. Cell number changes of drug action for 48h were examined by cell counting. Nuclear morphology was observed by DAPI staining. The mRNA levels and protein levels of apoptosis-associated genes of drug action for 48h were detected by RT-PCR and western blot respectively. Results With increasing of TMP concentration, the growth rate of 786-O cells decreased gradually, and apoptosis increased. The number of cells was significantly reduced and the nuclear shrinkage or swelling. Apoptosis related genes AIF and Caspase3 were significantly increased in the level of transcription and translation, no matter compared with blank control group or solvent control group(P<0.05). Conclusion This study confirmed TMP could decrease the proliferation and increase the apoptosis of 786-O cells. |
| keywords:Tetramethylpyrazine Cell proliferation Cell apoptosis Human renal cell carcinoma cell line |
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