| 高同型半胱氨酸血症兔致动脉粥样硬化的实验研究 |
| 投稿时间：2017-03-22 修订日期：2017-04-11 点此下载全文 |
| 引用本文：王晓萍,周明旺,颜春鲁,东红,杜改焕.高同型半胱氨酸血症兔致动脉粥样硬化的实验研究[J].医学研究杂志,2017,46(12):59-62 |
| DOI：
10.11969/j.issn.1673-548X.2017.12.016 |
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| 基金项目:甘肃省自然科学基金资助项目（1208RJZA187）；甘肃省高等学校科研项目（2013A-086） |
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| 中文摘要:目的 复制可靠的兔高同型半胱氨酸血症（HHcy）致动脉粥样硬化（AS）模型。方法 采用L-蛋氨酸皮下注射法建立HHcy家兔模型，并设生理盐水组为对照，比较两组注射前、注射后4、8、12周血清中Hcy、TC、TG、HDL-C、LDL-C值，实验结束后超声观察腹主动脉狭窄程度及病理切片观察AS形成的情况。结果 皮下注射L-蛋氨酸4周后血中Hcy明显高于正常组（P<0.05），HHcy模型形成；造模8、12周TC、TG水平显著升高（P<0.05）；造模12周模型组LDL-C水平显著升高（P<0.05）；造模12周后腹主动脉超声与病理均提示有明显的AS征象。结论 L-蛋氨酸皮下注射80mg/（kg·d）4周可建立HHcy模型，12周时动脉粥样硬化表现明显。 |
| 中文关键词:同型半胱氨酸 动脉粥样硬化 实验研究 动物模型 |
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| Experimental Study on High Homocysteine Induced Rabbit Atherosclerotic Lesions |
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| Abstract:Objective To replicate animal model of high homocysteine induced rabbit atherosclerotic lesions.Methods Rabbits were randomly divided into 2 groups:the model group and the control group. The model groups were injected L-methionine respectively, while the control group was given normal saline. The levels of Hcy in serum were analyzed before injection, 4 weeks, 8 weeks and 12 weeks after injection in each group. The formation of artherosclerosis was observed through type-B ultrasonic and pathological section. Moreover, the levels of Hcy, TC, TG, HDL-C and LDL-C in serum of each group were determined.Results After 4 weeks of injection, the levels of Hcy in serum in model groups were significantly higher than the control group (P<0.05). After 8 weeks and 12 weeks, the levels of TC and TG were significantly increased (P<0.05). After 12 weeks, the levels of LDL-C were significantly increased (P<0.05). After 12 weeks, the abdominal aorta has obvious signs of atherosclerosis with type-B ultrasonic and pathological section.Conclusion When administrated with L-methionine injection[80mg/(kg·d)], rabbit can be made into the homocystinemia model in the 4th weeks and artherosclerosis can be developed on the 12th week |
| keywords:Homocysteine Atherosclerosis Experimental research Animal models |
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