| 痛愈舒颗粒对寒凝血瘀型EMs大鼠子宫内膜窗口期胞饮小泡及LIF的影响 |
| 投稿时间：2017-04-24 修订日期：2017-05-10 点此下载全文 |
| 引用本文：姜北,丛慧芳,马遇春,徐乃伟.痛愈舒颗粒对寒凝血瘀型EMs大鼠子宫内膜窗口期胞饮小泡及LIF的影响[J].医学研究杂志,2018,47(2):115-119 |
| DOI：
10.11969/j.issn.1673-548X.2018.02.027 |
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| 基金项目:黑龙江省自然科学基金资助项目面上项目（H2015025） |
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| 中文摘要:目的 观察痛愈舒颗粒对寒凝血瘀型EMs大鼠窗口期子宫内膜胞饮小泡与白血病抑制因子（leukemia inhibitory factor，LIF）的影响。方法 依据实验要求选择健康大鼠，在动情周期制备寒凝血瘀型子宫内膜异位症大鼠模型。随机抽取造模后大鼠108只，平均分成6组，依次为模型4周组、模型8周组、给药低剂量组（中低组）、给药中剂量组（中中组）、给药高剂量组（中高组）和给药西药组（西药组）。采用免疫组化和电镜观察痛愈舒颗粒对寒凝血瘀型EMs大鼠窗口期子宫内膜胞饮小泡与LIF的影响。结果 EMs大鼠模型中，模型大鼠窗口期胞饮小泡的表达及LIF的表达均明显低于正常大鼠，差异有统计学意义（P<0.05），治疗用药后，与模型8周组比较，中药组胞饮小泡的成熟例数均增多，中高组表现明显，差异有统计学意义（P<0.05），且各中药组成熟胞饮小泡分布所占面积均增多，中高组表现更为密集饱满；与模型组比较，各中药组LIF的表达均明显升高，差异有统计学意义（P<0.05），其中以中药高剂量组疗效最好，差异有统计学意义（P<0.05），但西药组在两者表达上升高不明显。结论 痛愈舒颗粒治疗性给药可以提高寒凝血瘀型EMs模型大鼠窗口期子宫内膜组织中胞饮小泡与LIF的表达，提高内异症大鼠内膜容受性，增加模型大鼠受孕率。 |
| 中文关键词:痛愈舒颗粒 子宫内膜异位症 子宫内膜容受性 白血病抑制因子 |
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| Effects of the Tongyushu Granule on the Window Pinocytosis and Lif of the Endometrium of Ems Rats |
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| Abstract:Objective To observe the effect of Tongyushu granule on the EMs and the LIF of the rats with blood stasis due to cold accumulation.Methods According to the experimental requirements, the healthy rats were selected to prepare the model of blood stasis due to cold accumulation endometriosis. Randomly selected after modeling, 108 rats were divided into 6 groups, followed by the model 4 weeks group, 8 weeks model group, administration of low dose group, to medium dose group, high dose group of the Chinese medicine and administration of Western medicine group. The effects on the EMs of the endometrium and the LIF in the window period of blood stasis due to cold accumulation were observed by immunohistochemistry and electron microscopy.Results The EMs rat model, the expression of LIF in rat model and window period pinocytosis vesicles were significantly lower than those in normal rats(P<0.05),and the difference was statistically significant. After treatment, compared with model group of 8 weeks, the number of mature cases of Chinese medicine group of pinocytotic vesicles were increased, especially in the high dose group(P<0.05),and the difference was statistically significant. Compared with the model group, the expression of the traditional Chinese medicine group LIF increased significantly(P<0.05), and the difference was statistically significant. The high dose of Chinese medicine had the best curative effect(P<0.05),and the difference was obvious, but the western medicine group in the expression rise was not obvious.Conclusion The Tongyushu granule can improve the expression of endometrial tissue type of model rats with blood stasis due to cold accumulation EMs window vesicle and LIF, improve the endometrial receptivity of endometriosis in rats, and increase the pregnancy rate in rat model. |
| keywords:Tongyushu granule Endometriosis Endometrial receptivity LIF |
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