| 高尿酸血症大鼠肾脏抗氧化酶活力的实验研究 |
| 投稿时间：2017-05-15 修订日期：2017-06-20 点此下载全文 |
| 引用本文：任美玲,马玲,刘成刚,李冬戈,陈瑜,贾怀妙.高尿酸血症大鼠肾脏抗氧化酶活力的实验研究[J].医学研究杂志,2018,47(3):17-19 |
| DOI：
10.11969/j.issn.1673-548X.2018.03.006 |
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| 基金项目:国家重点基础研究发展计划（973计划）项目（2012CB722400） |
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| 中文摘要:目的 建成可靠的高尿酸血症（HUA）大鼠模型，研究HUA大鼠血清中氧化应激相关指标及肾功能的变化。方法 采用单一酵母喂饲和氧嗪酸联合酵母喂饲两种方法建成可靠的HUA大鼠模型，检测尿酸（UA）、尿素氮（BUN）、肌酐（CREA）、过氧化氢的含量及超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）的活力水平。结果 酵母组和氧嗪酸酵母组UA、BUN、CREA值均高于对照组（P<0.05）；酵母组和氧嗪酸酵母组SOD活力明显低于对照组（P<0.05）；相比对照和酵母组，氧嗪酸酵母组GSH-Px活力明显降低（P<0.05）；过氧化氢差异无统计学意义（P>0.05）。结论 单一酵母喂饲和氧嗪酸联合酵母喂饲均可建成可靠的HUA大鼠模型；两种喂饲方法诱导的HUA模型大鼠机体处于氧化应激状态，肾功能出现损害。 |
| 中文关键词:高尿酸血症 氧化应激 肾损伤 |
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| Experimental Study on Antioxidant Enzyme Activity in Kidney of Rats with Hyperuricemia |
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| Abstract:Objective HUA rat model was established to study the changes of serum oxidative stress and renal function in HUA rats. Methods A reliable HUA rat model was established by single yeast feeding and oxoxalic acid combined with yeast feeding.Detection of the vitality level of UA,CREA,BUN,SOD,GSH-Px and hydrogen peroxide was done. Results The UA, BUN and CREA values of yeast group and oxymatrine group were higher than those of control group (P<0.05).The activity of SOD in yeast group and oxazinium yeast group was significantly lower than that in control group (P<0.05).Compared with yeast and control, GSH-Px activity was significantly decreased (P<0.05), but there were no differences in hydrogen peroxide (P>0.05). Conclusion A reliable HUA rat model can be constructed by two methods:single yeast feeding and aerobic ammonium sulfate combined with yeast feeding.Two kinds of feeding methods induced HUA model rats were in oxidative stress state, renal function damage. |
| keywords:Hyperuricemia Oxidative stress Renal injury |
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