| 番茄红素对人骨肉瘤细胞顺铂增敏作用及机制研究 |
| 投稿时间：2016-12-18 修订日期：2017-02-28 点此下载全文 |
| 引用本文：柴旭泽,魏凯,王贵方.番茄红素对人骨肉瘤细胞顺铂增敏作用及机制研究[J].医学研究杂志,2018,47(4):167-170 |
| DOI：
10.11969/j.issn.1673-548X.2018.04.041 |
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| 中文摘要:目的 研究番茄红素（lycopene，LP）对人骨肉瘤MG-63细胞顺铂化疗敏感度的影响并初步探讨其可能的作用机制。方法 体外培养并取对数生长期人骨肉瘤MG-63细胞，设空白对照组、LP组（10μg/ml）、顺铂组（40μg/ml）和联合组[LP（10μg/ml）+顺铂（20μg/ml）]；药物干预48h后，采用噻唑蓝（MTT）比色法测定细胞增殖抑制率，流式细胞术（flow cytometry，FCM）检测细胞周期和细胞凋亡状况，反转录PCR（RT-PCR）法检测Bax mRNA、bcl-2 mRNA表达并计算Bax/bcl-2比值，蛋白免疫印迹（Western blot）法检测caspase-3蛋白表达。结果 联合干预组与顺铂组比较发现，联合干预组人骨肉瘤MG-63细胞增殖抑制率显著升高（P<0.05），细胞周期G0/G1期显著延长（P<0.05）、S期和G2/M期显著缩短（P<0.05，P<0.01），细胞凋亡率（apoptosis index，AI）显著升高（P<0.01），Bax mRNA表达明显上调、bcl-2 mRNA表达显著下调（P<0.05）且Bax/bcl-2比值显著升高（P<0.01），caspase-3蛋白表达上调（P<0.01）。LP组与空白对照组比较发现，LP组MG-63细胞周期明显改变（G0/G1期延长、G2/M期缩短），bcl-2 mRNA显表达著下调（P<0.05）、Bax/bcl-2比值显著升高（P<0.05），caspase-3蛋白表达显著上调（P<0.01）。结论 LP具有提高人骨肉瘤MG-63细胞顺铂敏感度的药理学作用，其机制可能与LP能够阻滞细胞周期以及调节凋亡相关调控基因和蛋白表达有关。 |
| 中文关键词:番茄红素 骨肉瘤 顺铂 增敏作用 凋亡 |
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| Efficacy Enhancing Effect of Lycopene on Human Steosarcoma Cells Treated by Cisplatin |
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| Abstract:Objective To investigate the efficacy enhancing effect and mechanism of lycopene (LP) on human steosarcoma MG-63 cells treated by Cisplatin. Methods The human osteosarcoma MG-63 cells in logarithmic growth phase was treated with LP (10μg/ml), Cisplatin (40μg/ml) and LP (10μg/ml)+Cisplatin (20μg/ml), and blank group was set. The cellular growth inhibition rate was calculated by MTT. Cell cycle and apoptosis rate were analyzed by flow cytometry(FCM). The expression of Bax mRNA and bcl-2 mRNA were detected by RT-PCR and the ratio of Bax/bcl-2 was calculated. The expression of Caspase-3 protein was detected by western blotting. Results Compared with Cisplatin group, the cellular growth inhibition rate of human osteosarcoma MG-63 cell in LP+Cisplatin group was significantly increased(P<0.05). The cell cycle was arrested at G0/G1 phase(P<0.05). The apoptosis rate was significantly increased(P<0.01). The expression of Bax mRNA was increased, while the expression of bcl-2 mRNA was significantly decreased(P<0.05) and the ratio of Bax/bcl-2 was significantly increased(P<0.01). The expression of Caspase-3 protein was significantly increased(P<0.01). Compared with blank group group, the G0/G1 phase in cell cycle of LP group was increased and the G2/M phase was decreased, and the expression of bcl-2 mRNA was significantly decreased(P<0.05). The ratio of Bax/bcl-2 was significantly increased (P<0.05). The expression of Caspase-3 protein was significantly increased (P<0.01). Conclusion LP can effectively enhance the effect on human liver cancer MG-63 cells treated by Cisplatin, which perhaps was related to its effects of altering the cell cycle and the expression of apoptosis-related genes and proteins. |
| keywords:Lycopene Osteosarcoma Cisplatin Enhancing effect Apoptosis |
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