| miRNA-145调控靶基因c-Myc抑制胃癌增殖、侵袭 |
| 投稿时间：2017-07-28 修订日期：2017-09-12 点此下载全文 |
| 引用本文：彭伟辉,黄江生,段伦喜,张磊屹,左仲坤,唐腾龙.miRNA-145调控靶基因c-Myc抑制胃癌增殖、侵袭[J].医学研究杂志,2018,47(5):60-64 |
| DOI：
10.11969/j.issn.1673-548X.2018.05.015 |
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| 基金项目:湖南省科学技术厅基金资助项目（2016jc2048） |
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| 中文摘要:目的 探索microRNA145（miR-145）对胃癌细胞增殖活性的影响，及与其靶基因c-Myc的调控关系。方法 选取人胃癌细胞株SGC-7901作为研究对象，并向细胞株内转染miR-145模拟物和miR-145阴性对照物，荧光显微镜观察、Q-PCR检测转染后细胞株miR-145的变化，并通过细胞软琼脂克隆增殖实验和MTT实验检测转染后SGC-7901细胞株的增殖能力、Transwell侵袭小室检测细胞侵袭能力。Western blot法检测转染后SGC-7901细胞株中c-Myc蛋白的表达情况。结果 转染后，miR-145模拟物组中miR-145的表达明显高于miR-145阴性对照和空白组，转染miR-145模拟物后SGC-7901细胞增殖能力显著降低（P<0.05），c-Myc蛋白表达也明显较低（P<0.05）。结论 miR-145能通过抑制靶基因c-Myc的表达，抑制胃癌细胞的增殖与侵袭能力。总之，miR-145可能在胃癌中发挥抑癌基因的作用，是胃癌潜在的早期诊断指标和治疗靶点。 |
| 中文关键词:胃癌 miR-145 c-Myc 增殖 |
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| miR-145 Inhibite Tumor Proliferation and Invasion in Gastric Cancer by Targeting c-Myc |
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| Abstract:Objective To investigate the effect of miR-145 on proliferation of gastric cancer, And its relationship with target gene c-Myc.Methods Human gastric cancer cell line SGC-7901 was selected as study object. miR-145 mimic and miR-145 NC were synthesised and transfected into SGC-7901 cells. Q-PCR was used to detect the relative expression levels of miR-145. The effects of miR-145 on proliferation and invasion were detected by colony formation and MTT assay and Transwell invasion chambers. The expression of c-Myc were detected by Western blot.Results miR-145 were successfully transfected into SGC-7901 cells. The number of colony-forming units was obviously lower than the miR-145 NC group and Normal group (P<0.05). The expression of c-Myc protein in miR145 mimic group were significantly lower than miR-145 NC group and Normal group (P<0.05).Conclusion Therefore, we concluded that miRNA-145 can inhibit cell proliferation and invasion, and the partial mechanism may relate to the inhibition of the expression of c-Myc protein. In brief, miRNA-145 may play a role of cancer suppressor gene and it may become a new early diagnosis mark and therapy target in gastric cancer. |
| keywords:Gastric cancer miR-145 c-Myc Proliferation |
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