| 不同剂量FTY720对颅脑损伤大鼠神经细胞凋亡的抑制作用 |
| 投稿时间：2017-10-10 修订日期：2017-11-07 点此下载全文 |
| 引用本文：蔡建勇,吴艳,林群,巴华君,陆川,陈献东,陈茂华,孙军.不同剂量FTY720对颅脑损伤大鼠神经细胞凋亡的抑制作用[J].医学研究杂志,2018,47(8):96-99,108 |
| DOI：
10.11969/j.issn.1673-548X.2018.08.022 |
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| 基金项目:浙江省实验动物科技计划项目（2014C37029） |
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| 中文摘要:目的 揭示不同剂量FTY720对颅脑损伤大鼠海马组织神经细胞凋亡的影响及相关机制。方法 将175只大鼠随机分成假手术组、模型组、小剂量组、中剂量组和大剂量组，每组35只，再根据2、6、12h、1、2、3和5天7个时间点分成7个亚组，每组5只。采用Feeney自由落体损伤装置建立大鼠颅脑损伤模型。小剂量组、中剂量组和大剂量组大鼠分别按0.5、1和2mg/kg剂量予FTY720腹腔注射，其他组大鼠腹腔注射1ml生理盐水。检测各时间点海马组织凋亡神经细胞、caspase-3蛋白活性和白介素-1β、肿瘤坏死因子-α及白介素-6浓度。结果 模型组大鼠海马组织凋亡神经细胞比例、caspase-3蛋白活性和白介素-1β、肿瘤坏死因子-α和白介素-6浓度较假手术组显著升高，小剂量组、中剂量组和大剂量组大鼠海马组织上述指标均较模型组显著下降，且作用效果呈现剂量依赖性。结论 FTY720可能通过抑制中枢炎性反应从而减少颅脑损伤大鼠海马组织神经细胞凋亡，且呈现剂量依赖性。 |
| 中文关键词:FTY720 大鼠 炎性反应 神经细胞凋亡 颅脑损伤 |
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| Inhibitory Effect of Different Dosages of FTY720 on Neuronal Cellular Apoptosis of Rats with Traumatic Brain Injury |
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| Abstract:Objective To investigate the effect of different dosages of FTY720 on neuronal cellular apoptosis of hippocampus tissues in rats with traumatic brain injury and the related mechanism. Methods Totally 175 rats were randomly assigned to sham-operation group, model group, small dosage group, medium dosage group and large dosage group with 35 rats in each group. According to 7 time points, i.e. hour 2, hour 6, hour 12, day 1, day 2, day 3 and day 5, each group was divided to 7 subgroups with 5 rats in each subgroup. Feeney free fall injury device was utilized to establish rat craniocerebral injury model. Rats in small dosage group, medium dosage group and large dosage group were administrated intraperitoneally with 0.5, 1 and 2 mg/kg FTY720 respectively. Rats in other groups were given an intraperitoneal injection of normal saline. At each time point, the apoptotic neuronal cells, activity of caspase 3 protein and concentrations of interleukin-1beta, tumor necrosis factor-alpha and interleukin-6 were determined in hippocampus tissues. Results Compared with sham-operation group, the apoptotic neuronal cells, activity of caspase 3 protein and concentrations of interleukin-1beta, tumor necrosis factor-alpha and interleukin-6 in hippocampus tissues were obviously elevated in model group. The preceding variables in hippocampus tissues were statistically significantly lower in small dosage group, medium dosage group and large dosage group than in model group in a dose-dependent manner. Conclusion FTY720 could inhibit inflammation in central nervous system and subsequently suppress neuronal cell apoptosis of the rat hippocampus tissues following trauma brain injury in a dose-dependent manner. |
| keywords:FTY720 Rat Inflammatory reaction Neuronal cell apoptosis Traumatic brain injury |
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