| 太白楤木总皂苷对小鼠急性酒精性肝损伤的干预作用 |
| 投稿时间：2018-01-03 修订日期：2018-01-11 点此下载全文 |
| 引用本文：范妤,郭东艳,郑蓓.太白楤木总皂苷对小鼠急性酒精性肝损伤的干预作用[J].医学研究杂志,2018,47(10):54-57 |
| DOI：
10.11969/j.issn.1673-548X.2018.10.015 |
| 摘要点击次数: 916 |
| 全文下载次数: 647 |
|
| 基金项目:国家自然科学基金青年基金资助项目（81703925）；陕西省中医药管理局基金资助项目（15-ZY002）；陕西省教育厅基金资助项目（16JK1218）；陕西省中药基础与新药研究重点实验室开放基金资助项目（2017KF03） |
|
| 中文摘要:目的 探讨太白楤木总皂苷对小鼠急性酒精性肝损伤的保护作用及其机制。方法 将健康昆明小鼠60只随机分为空白组（生理盐水）、模型组（生理盐水）、阳性对照组（联苯双酯，125mg/kg）、太白楤木总皂苷高、中、低剂量组（400、200、100mg/kg），每组10只，雌雄分开饲养，灌胃给药。除空白组外，其余各组每日上午给予56°北京二锅头（10mg/kg）灌胃，下午给予干预治疗，连续7天。末次给药8h后，检测小鼠肝脏指数，血清及肝组织中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）含量，肝组织中超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量，HE染色观察肝组织病理改变。结果 与模型组比较，太白楤木总皂苷不同浓度治疗组均能够降低酒精性肝损伤小鼠血清及肝组织中ALT、AST水平（P<0.01），升高肝组织SOD活性（P<0.01），降低MDA含量（P<0.05）；肝组织HE染色显示，太白楤木总皂苷可明显减轻小鼠肝损伤的病变程度。结论 太白楤木总皂苷对急性酒精性肝损伤小鼠具有一定的保护作用，其作用机制可能与对抗自由基生成，抑制氧化应激来实现抑制抗氧化应激有关。 |
| 中文关键词:太白楤木总皂苷 急性酒精性肝损伤 氧化应激 |
| |
| Effect of Total Saponin of Aralia Taibaiensison Acute Liver Injury Induced by Alcohol in Mice |
|
|
| Abstract:Objective To study the protectioneffect of Total Saponin of Aralia Taibaiensis(TSAT) on liver injury induced by alcohol in miceand explore itspossible pharmacological mechanisms. Methods A total of 60 mices were randomly divided into normal group,model group,bifendate group (125mg/kg) and TSAT high dose,middle dose,and low dose groups(400、200、100mg/kg).10 mices in each group were fed and reared, and the medicine was given to the stomach.Except the normal group, other groups were given 56°Beijing Erguotou (10mg/kg) in the morning, the medicine group were given the corresponding medicine by gavage in the afternoon once a day for 7 days. 8 Hours later,blood and liver samples of mice were collected for the calculation of liver indexes of mice ineach group and the detection AST and ALT levels in serum and liver tissues as well as SOD activity and MDA in liver tissues.Histopathological changes of liver were observed by HE staining. Results Compared with model group, TSAT could significantly reduce the liver index in mice (P<0.05), reduce ALT and AST levels in serum and liver tissues (P<0.01),increase the activities of SOD (P<0.01)and decrease the MDA content (P<0.05) in liver tissues.Pathological changes of the mice liver were observed by HE staining. Conclusion TSAT may have protection effect on alcohol-induced liver injury in mice,and the mechanism may be associated with the regulation of oxidative stress in the liver. |
| keywords:Total Saponin of Aralia Taibaiensis Acute alcohol-induced liver injury Oxidative stress |
| 查看全文 查看/发表评论 下载PDF阅读器 |
