| 小鼠c-Kit和Sca-1阳性干细胞的多器官年龄分布特征 |
| 投稿时间：2018-02-05 修订日期：2018-03-17 点此下载全文 |
| 引用本文：周密,李端端,吕定超,石山慧,马秀霞,李燕楠,陈思宇,左琳.小鼠c-Kit和Sca-1阳性干细胞的多器官年龄分布特征[J].医学研究杂志,2018,47(12):50-54 |
| DOI：
10.11969/j.issn.1673-548X.2018.12.013 |
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| 基金项目:国家自然科学基金资助项目（81200120）；山西省青年基金资助项目（面上项目）（201601D202106）；山西省回国留学人员科研基金资助项目（2014-036） |
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| 中文摘要:目的 本研究旨在研究c-Kit和Sca-1两种干细胞亚型在小鼠多脏器的年龄分布规律，为后续进行相关组织的干细胞抗损伤研究奠定基础。方法 选取C57/BL6雄性成年小鼠（3月龄、6月龄、9月龄），分离其骨髓、肝脏、肾脏、肺及胰腺组织，采用组织块酶解法分选各脏器中直径小于30μm的细胞，用CD45、c-Kit及Sca-1荧光抗体标记细胞，流式细胞仪记录CD45-c-Kit+以及CD45- Sca-1+细胞百分比。结果 在小鼠骨髓中，CD45-c-Kit+细胞数显著多于CD45- Sca-1+（P<0.05）；而在肝、肾、肺和胰腺组织中，CD45- Sca-1+的细胞数量远多于CD45-c-Kit+干细胞（P<0.05）。除胰腺组织，9月龄CD45- Sca-1+的细胞数目较3、6月龄有所升高外，其他组织中干细胞的年龄分布趋势差异不显著（P>0.05）。结论 CD45-c-Kit+和CD45- Sca-1+两种干细胞亚型在小鼠多脏器中均有存在，尤其是数量上占据优势的CD45- Sca-1+干细胞可能在多数器官的发育及修复中发挥重要作用。 |
| 中文关键词:干细胞 c-Kit Sca-1 分布 |
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| Age Distribution of c-Kit and Sca-1 Positive Stem Cells in Multi-organs of Mice |
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| Abstract:Objective To investigate the age distribution of c-Kit and Sca-1 positive stem cells in multi-organs which can provide more detailed information for the further researches on anti-damage of stem cells in different tissues. Methods C57/BL6 male mice (3months, 6months and 9months) were grouped randomly. The cells less than 30μm were isolated by tissue enzyme hydrolysis from bone marrow, liver, kidney, lung and pancreas. These cells were labeled with CD45, c-Kit and Sca-1 fluorescent antibody. The percentage of CD45-c-Kit+ and CD45- Sca-1+stem cells can be calculated by flow cytometry. Results The results indicated that the amount of CD45-c-Kit+ stem cells was larger than CD45- Sca-1+ stem cells in bone marrow (P<0.05); However, in liver, kidney, lung and pancreas, the percentage of CD45- Sca-1+ stem cells was higher than CD45- c-Kit+cells (P<0.05). In pancreas, the percentage of CD45- Sca-1+ in 9months was higher than that of CD45- c-Kit+ in 3months and 6months group (P< 0.05). In other organs, there was no significant difference of CD45- c-Kit+ and CD45- Sca-1+ stem cells between different age groups. Conclusion These results suggested that CD45- c-Kit+ and CD45- Sca-1+ stem cells located in many main organs of mice. Especially, CD45- Sca-1+ stem cells superior in numbers may play an important role in multi-organs' development and repair. |
| keywords:Stem cell c-Kit Sca-1 Distribution |
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